Intermediate

Part:BBa_K3221100:Design

Designed by: lingfeng mao   Group: iGEM19_SCU-China   (2019-09-30)


GAL1-T7 + cns1+CYC1 + CYC1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 624
    Illegal XbaI site found at 2169
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 624
    Illegal NheI site found at 545
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 624
    Illegal BamHI site found at 609
    Illegal XhoI site found at 2163
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 624
    Illegal XbaI site found at 2169
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 624
    Illegal XbaI site found at 2169
    Illegal AgeI site found at 70
    Illegal AgeI site found at 699
    Illegal AgeI site found at 2232
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We have done codon optimization in saccharomyces cerevisiae and mutations for the remove of the restriction sites.

Source

the part is contructed by our team

References

Xia Y , Luo F , Shang Y , et al. Fungal Cordycepin Biosynthesis Is Coupled with the Production of the Safeguard Molecule Pentostatin[J]. Cell Chemical Biology, 2017:S2451945617303276.